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1 article(s) from Sheikhi Mehrabadi, Fatemeh

Multivalent dendritic polyglycerolamine with arginine and histidine end groups for efficient siRNA transfection

Fatemeh Sheikhi Mehrabadi,
Hanxiang Zeng,
Mark Johnson,
Cathleen Schlesener,
Zhibin Guan and
Rainer Haag

Beilstein J. Org. Chem. 2015, 11, 763–772, doi:10.3762/bjoc.11.86

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Graphical Abstract

Scheme 1: Synthesis of multivalent arginine and histidine functionalized dPG-NH₂ 50%. The depicted dPG-NH₂ re...

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Figure 1: Agarose gel electrophoresis retardation assay of AAdPGs/siRNA polyplexes. (A) dPG-13Arg13His, (B) d...

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Figure 2: Size measurements of dPG-NH₂ 50% and AAdPGs/siRNA complexes. Intensity distributions of all polyple...

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Figure 3: The result of MTT assay on a NIH 3T3 cell line transfected with AAdPG, dPG-NH₂ 50%, and 90%/siRNA p...

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Figure 4: Cell viability versus transfection efficiency of dPG-8Arg30His and dPG-NH₂ 90% at N/P ratio 30.

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Figure 5: Summary of transfection results versus viability of AAdPGs with various Arg and His composition rat...

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Figure 6: Confocal images of NIH 3T3 cells treated with Cy3-siRNA/vector complexes: (A) naked siRNA, (B) lipo...

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Published 13 May 2015

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